In ordinary Catholic devotional use outside the Spiritual Exercises, Anima Christi is most commonly prayed silently after receiving communion, functioning as a personal meditation on union with Christ during the period immediately following reception rather than as public liturgical text spoken aloud during the Mass itself. This distinguishes it functionally from prayers like the Collect for Purity, which are spoken by or on behalf of the whole congregation as part of the fixed liturgical order, since Anima Christi's use is largely a matter of personal devotional custom layered on top of the official liturgy rather than a required element of it. Its intensely physical, almost visceral imagery, being washed in Christ's blood or hidden within his wounds, has made it a frequent example cited in discussions of affective, emotionally engaged medieval and early modern Catholic devotion, contrasted with more restrained or doctrinally worded prayers from the same general period.
Musical settings of the prayer, particularly a well-known melody often used in contemporary Catholic worship, have also given Anima Christi a life as a hymn sung congregationally, in addition to its older role as a text of silent personal recitation, broadening its use well beyond the private post-communion meditation Ignatius originally recommended.