Ambrose's tenure defined how a Western bishop could face an emperor without breaking from him. In 386 the empress-regent Justina, who favored the Homoian Arian position, ordered Ambrose to surrender a Milan basilica for Arian worship. He refused and occupied the church with his congregation for days, and it was during this standoff that he introduced antiphonal psalm-singing, dividing the congregation into responsive choirs to sustain morale, a practice historians credit with shaping what later tradition calls Ambrosian chant.
The court backed down. Four years later, after the massacre of thousands of civilians at Thessalonica ordered by Emperor Theodosius I in reprisal for a riot, Ambrose wrote privately urging repentance and then, when the emperor next presented himself at Milan for the liturgy, refused him communion until he had done public penance. Theodosius complied, removing his imperial regalia and standing among the penitents, an episode later read as an early statement that the emperor was a son of the church, not its master.
Ambrose's preaching on the Old Testament and on the nature of the church, delivered while he was also negotiating politics of this scale, reached a young professor of rhetoric named Augustine, who had come to Milan skeptical of the Manichaean religion he had followed for years. Augustine described in the Confessions how Ambrose's allegorical reading of scripture removed the intellectual objections that had kept him from the Nicene faith. Ambrose baptized Augustine at the Easter vigil of 387. He was declared one of the four original Doctors of the Church, alongside Jerome, Augustine and Gregory the Great.